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48 h after entering the low-pressure chamber, the rats were subjected to anesthesia through an intraperitoneal injection of 10% urethane at 1.5 g/kg, with placement in the supine position for fixation

Subsequently, supplementary B12 may be necessary

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These happened due to neuronal atrophy and enhanced density of Nissl bodies and neurons of the hippocampus (70, 72)

Depletion of the GM leads to the loss of its mediated negative feedback control on BAs synthesis, reduced signaling to the FXR, elevated hepatic BAs concentrations, impaired biliary barrier function, and ultimately exacerbation of CLI [74]

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